Quantitation Of Protein

Introduction In today’s world we reckon on pharmaceutical drugs more than ever and production of these drugs standardized insulin or human growth hormone, require quantitation of protein. By execute experiments on quantitation of protein we give the axe discover faster methods or new shipway to achieve this and as well as see which method is the most cost-effective. every(prenominal) company wants an improvement over the former(a) companies, so if they stop find a faster or more efficient way of quantitating protein, they can bring on more money comp atomic number 18d to the other companies. This lab uses a conjecture of spectrophotometry and an understanding of the Beer-Lambert law; it also uses three hindrances utilise to quantitate protein. Spectrophotometry uses a device called a Spectrophotometer which can measure the absorbance of wakeful at different wavelengths in a resoluteness. The Beer-Lambert’s law relates the absorbance to the compactness of the solution, the pathlength of the cuvette and the extinction coefficient (A=ecl). Three stresss used to quantitate protein in this lab are Coomassie Blue, Lowry and UV direct. Coomassie blue is a dye binding method, where it binds to the protein fork out in the solution and changes the discolour of the solution which is then metrical by the spectrophotometer. Lowry assay is where the cupric ions react with the peptide bonds under saltlike conditions ever-changing the colour of the solution which can then be measured. The UV direct assay measures the amount of UV light absorbed by the solution. The documental of this lab is to find concentrations of proteins using standards that were made for each assay; hoping to see similar concentrations for all proteins. Methods (Carleton University, 2009) Results In figure 1 you can see the absorbance value across different wavelengths for the p-np solution. The measurable thing to note is at what wa velength the graph peaks at. Table 1: Absor! bance and Extinction coefficient values for changing concentrations of...If you want to get a full essay, order it on our website: BestEssayCheap.com

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